Laboratory
TcLand Expression’s Scientific and Technical teams have built a Laboratory based on State of The Art technologies for gene expression and repertoire analyses.
Our Laboratory is equipped with Laboratory Information Management System (LIMS) SQL*LIMS® Software and enables:
- Automation of Standard Operating Procedures –SOPs- that must be followed during the QA/QC process
- Full traceability of all samples from entry to release, via processing, storage or destruction
- Elimination of inefficiency and transcription errors rates and improvement of compliance with agency regulations in terms of Quality
Available techniques:
- Study design support
- Standardized sampling for gene expression (PAXgene™, Trizol, RNAzol…)
- Type II Microbiological Safety Cabinet (MSC)
- defrost DMSO frozen cells
- total RNA blood extraction PAXgene™ (individual vial, semi automated 96 PAXgene™ plates)
- Sorbonne Safety Cabinet, total RNA extraction
- Trizol
- RNA later
- RNAzol
- Dry pellet
- Biopsies (organs, tissues)
- RNA isolated Quality Control, NanoDrop® ND-1000 and Agilent 2100 Bioanalyser
- Quantity
- Purity
- Integrity
- RT and SMART ™ cDNA linear amplification
- Automated AB 7900HT TaqMan® qPCR
- AB 3730 fragment analyses
- Data management
- Animal models used are human, mouse, rat and primate.